| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006675 |
| Chromosome: | chromosome 12 |
| Location: | 7571628 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556050 | PRPL9 | Chloroplast ribosomal protein L9; (1 of 1) K02939 - large subunit ribosomal protein L9 (RP-L9, MRPL9, rplI) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGACGGCATGTGGGGAGGGCAGAAGCGGCAGCGGCGCAACACGGAGGC |
| Internal bar code: | CATTGTACATACATTACGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 61.1111 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCCTCACACAAAGCAC |
| Suggested primer 2: | GAGATTGCCGACGCCATCTA |