Insertion junction: CLIP2.006690_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006690
Chromosome:chromosome 14
Location:1949591
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g620600 PHC2,FAP202 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGGATAGCCTGCTTGCAACTTGCTGTCAAATCCGGTGGTGGCGCTACG
Internal bar code:TCTAAGTGCGGGCCTTGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1034
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGAACCACCTCTGTGACA
Suggested primer 2:GTGGAGATAGGGCAGCAGTC