| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006708 |
| Chromosome: | chromosome 9 |
| Location: | 1059247 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401950 | (1 of 8) 2.7.11.12 - cGMP-dependent protein kinase / PKG II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGGCGCTGCCACTGGGCAGGCGGCAGCCGCACAGCAGCTGCCGTCA |
| Internal bar code: | GTTTCTCCGGTCGAGCAGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 3.0303 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTAACGCTGACACTGGT |
| Suggested primer 2: | TTACGTCGAACGACCCGTAC |