Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.006712 |
Chromosome: | chromosome 1 |
Location: | 115477 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g000800 | CGL136 | Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGTAGTCGGCCGCGCCATACTCGCGGTGCAACATAACAATCACTATC |
Internal bar code: | AGGCAATGCGAATATTTTGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3120 |
LEAP-Seq percent confirming: | 88.3721 |
LEAP-Seq n confirming: | 38 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCAACAGACGCACTTTTCA |
Suggested primer 2: | AGCACCTTCAGACCCATTCG |