| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006716 |
| Chromosome: | chromosome 14 |
| Location: | 3389599 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629920 | AMT1B,AMT2 | (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP); Ammonium transporter (AMT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGAAAGAAAGCCCCCGCGCCATGACTGTGACGGCGGCACTGCAAAGT |
| Internal bar code: | TGAATCGAAGCGACCTCGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1209 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGCGTTGTTGAGCTTGC |
| Suggested primer 2: | CAGCAGCACGATGATTGACG |