| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006727 |
| Chromosome: | chromosome 8 |
| Location: | 1545244 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365200 | CSE8,FAP321 | Coiled-Coil Flagellar Associated Protein 321 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCGTCGCCCGCGCCTCCCAGCCCCAGCCCGTCGCCTCCGGCGCCTC |
| Internal bar code: | GTTTTGTATACGGGTGCTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1954 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAGAAGGAGGCGAAAC |
| Suggested primer 2: | ACGTCTCGAGCGAAAACACT |