| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.006731 |
| Chromosome: | chromosome 9 |
| Location: | 5007311 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405550 | TIM21 | Mitochondrial inner membrane translocase subunit; (1 of 1) K17796 - mitochondrial import inner membrane translocase subunit TIM21 (TIM21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGGGTTTCGGGGGTGAGCACGCAGAACCGGAGTGGGTGCGCGAGGA |
| Internal bar code: | TCTCGAGAAAAGGCTTATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGAGGCACGTATCACCC |
| Suggested primer 2: | CTTTGGAGGCTTCGTTTGGC |