| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006737 |
| Chromosome: | chromosome 13 |
| Location: | 3883685 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589650 | (1 of 1) PF08424 - NRDE-2, necessary for RNA interference (NRDE-2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCGCGTGAGGGAGTGGCCCCGATGCGGGCAGCGTGCATGCAGTACAG |
| Internal bar code: | CATTCTAAATGGCTACCGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAGTACAACACAGCAGT |
| Suggested primer 2: | ATGCACACAGTGAAGAGCGA |