Insertion junction: CLIP2.006740_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.006740
Chromosome:chromosome 6
Location:7427703
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g300500 ADCY1,CYA18 Adenylyl cyclase; (1 of 1) PTHR11347//PTHR11347:SF132 - CYCLIC NUCLEOTIDE PHOSPHODIESTERASE // PDE1C, ISOFORM E CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCCCTGCCACCGCCGGCAGCGGCTGCTGCTGTCCCGACGGCAGCGGAG
Internal bar code:TTATTTACAATATTTTTACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1743
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGTCAACTCCATCGCACA
Suggested primer 2:GCATTAATCACGTCGCGCTT