| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006745 |
| Chromosome: | chromosome 6 |
| Location: | 5294356 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284600 | RBD2 | (1 of 1) PTHR17130//PTHR17130:SF22 - MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25 // SUBFAMILY NOT NAMED; rubredoxin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGTGACCTGGCGATAAAAGCGTCGGCTGCCTATGGCGCCACGCGACC |
| Internal bar code: | TCATGGAAAACTTGCTTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCCTAGGCTGTGCAAG |
| Suggested primer 2: | AATTTGCTTGGCTGGCAGTG |