Insertion junction: CLIP2.006746_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006746
Chromosome:chromosome 3
Location:5552441
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185250 SSS2,SSII (1 of 1) IPR001296//IPR011835//IPR013534//IPR015421 - Glycosyl transferase, family 1 // Bacterial/plant glycogen synthase // Starch synthase, catalytic domain // Pyridoxal phosphate-dependent transferase, major region, subdomain 1; Soluble starch synthase II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCTACTCGGTCTTCAACTCCAACCAAGAGGTGCGCGGCGGACAGCTGG
Internal bar code:GTTATTATAAAAGCATTGTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1660
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGCGTGGAAGTAGCCCA
Suggested primer 2:CGCTGGAGTCGGAGATGTAC