| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006748 |
| Chromosome: | chromosome 11 |
| Location: | 846789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467644 | HSP101,CLPB1 | (1 of 3) K03695 - ATP-dependent Clp protease ATP-binding subunit ClpB (clpB); ClpB chaperone, Hsp100 family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACCGCATCAAGCAGGTTCGCGCGGCCGCGCCCTCCGACGCCATGCTG |
| Internal bar code: | TCATTGGTCAGGTCAGCAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3762 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCTCCACCTCGTCAAA |
| Suggested primer 2: | GAGGCCACTGCAGATGAAGT |