Insertion junction: CLIP2.006752_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006752
Chromosome:chromosome 1
Location:5212190
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g036350 PMA1 (1 of 5) K01537 - Ca2+-transporting ATPase (E3.6.3.8); Related to plasma membrane P-type and chloroplast type calcium ATPases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACTTGCACCGCATCATGAAAACCACAGAGTCGTCAGCAACTAGCTAG
Internal bar code:CCACATCTGTAAGGGACTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2809
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CATATCGTCACAGCCACCCA
Suggested primer 2:CATGGAGAGGGGAGTGGGTA