| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006762 |
| Chromosome: | chromosome 17 |
| Location: | 3822828 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGCCCCAGCAGCAGCACGGGCGGCGACGAGCTGGTTGTGGTGGGGC |
| Internal bar code: | CTAATCTCGAGACTCGACGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2878 |
| LEAP-Seq percent confirming: | 48.8889 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTTGCCCAATCCTGCA |
| Suggested primer 2: | ACCTGCTATCAGAACCCCCT |