| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006763 |
| Chromosome: | chromosome 1 |
| Location: | 2408860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013801 | SXD1,TCY1 | (1 of 1) 5.5.1.24 - Tocopherol cyclase; Tocopherol cyclase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGGCAAACGCTGCATAGCAGACACGCGAGGGTTTCAAGGGCGTCCA |
| Internal bar code: | TTCCCGGCATCGTGGGAGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1537 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACAGACTTGGTGGCGA |
| Suggested primer 2: | TACCCAAATCCACTCGCACC |