Insertion junction: CLIP2.006785_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.006785
Chromosome:chromosome 9
Location:1475788
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399914 SSA8 cilia-sensing, structure and/or assembly; (1 of 1) K10766 - alkylated DNA repair protein alkB homolog 4 (ALKBH4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAAGAAATTTCCTAGAAAAACTGACCGCGCCAGAACCGGTACTACCATT
Internal bar code:TGGTTCTCCGTCAGCTGCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2707
LEAP-Seq percent confirming:11.3208
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCCTCAGGAGTCAAAGAC
Suggested primer 2:CACAAAGTCCAACACCAGCG