Insertion junction: CLIP2.006788_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006788
Chromosome:chromosome 12
Location:1114627
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTCACCAGGCAGTAAGGCACCCAAAGACTGCGCGCATACACACACAT
Internal bar code:TTACTTACAGGAGGCGTAAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:797
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGCGGATCTGGATCTC
Suggested primer 2:CAAAACCCCTGCCAACTTCG