Insertion junction: CLIP2.006806_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006806
Chromosome:chromosome 12
Location:7436782
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g557050 CPLD8,YGG2 Conserved expressed protein with YGGT domain; (1 of 2) K02221 - YggT family protein (yggT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCCCTCTTCCATCACCGCCCATGCACGCAACATCAACTCAGCCCCCAC
Internal bar code:CGGGACCCGGATGGGGCGACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:65
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCAAGGATATCGGCTCG
Suggested primer 2:TGCATACACACACACGCTCT