Insertion junction: CLIP2.006810_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.006810
Chromosome:chromosome 9
Location:4065983
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399030 HPD2 (1 of 1) K00457 - 4-hydroxyphenylpyruvate dioxygenase (HPD, hppD); 4-hydroxyphenylpyruvate dioxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAACACAAGAGCCCACACATGCCCATATGCCCACACGCGCACGTGCG
Internal bar code:CGCGGGGAGGGGGAGAAACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGAACGCATTGGCTGTAT
Suggested primer 2:TAGGCATGAGCAACCTGTGG