Insertion junction: CLIP2.006815_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.006815
Chromosome:chromosome 6
Location:5256342
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g284250 LHCBM8 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGCTGTATGTCAAAATTGAAGGCACAACATTGTGTGTGTGCATGTGG
Internal bar code:TTTCCGGCGTGGATCAGACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTGAGCTGAAGGTGAAG
Suggested primer 2:GCCTAACACTTGGCCTGGAT