Insertion junction: CLIP2.006816_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.006816
Chromosome:chromosome 11
Location:1517664
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g468750 CPLD48 Conserved in the Plant Lineage and Diatoms; (1 of 3) PF07386 - Protein of unknown function (DUF1499) (DUF1499) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACACACGCCTGTGCAGACAGGGTGATGGCAGCACTCCTACCAAGTTG
Internal bar code:GCTTAAGATCAACACGTGCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2822
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGGAACATGTTAGCGCG
Suggested primer 2:GGAGCTCATTGAAGGACGCT