| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006821 |
| Chromosome: | chromosome 12 |
| Location: | 1778671 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485750 | CGL128 | (1 of 1) PTHR13420//PTHR13420:SF5 - UNCHARACTERIZED // UPF0235 PROTEIN; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGTATGCTCGTTCGTGTCCACGATGTAAGCCCCGTCCGTTCGCCGCC |
| Internal bar code: | CTAATAGTAGGGTGATTGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1722 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCAGCACGAACTGGACA |
| Suggested primer 2: | CCTCTTCCTCCTCCTCCTCC |