| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006835 |
| Chromosome: | chromosome 11 |
| Location: | 648910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467613 | (1 of 1) IPR000742//IPR001881 - EGF-like domain // EGF-like calcium-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGAGGGTGCAATTGGAGGAGGAGAGCAGCACCGCCCTGCGCGAGGTG |
| Internal bar code: | TATGAACTTCTGTCTCAGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1256 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTCTCGGGTGAGTTGGA |
| Suggested primer 2: | ATCAGGCCTGTGTGGGAAAG |