Insertion junction: CLIP2.006846_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006846
Chromosome:chromosome 6
Location:4903043
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre06.g280650 CGL59,Y3IP1 Chloroplast Ycf3 interacting protein involved in Photosystem I biogensis intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCACCTCAATATCCTGCGCGCGGCGCTCGCGGGCGCGCGGGTCCTCA
Internal bar code:GTTAGATGCTCGGGCAGATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1578
LEAP-Seq percent confirming:32.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCACAAAATGAGCGCGCA
Suggested primer 2:CCGCAAATGCAACAACAGGA