Insertion junction: CLIP2.006850_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006850
Chromosome:chromosome 3
Location:6066141
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g190150 CGL1 (1 of 2) PF11833 - Protein of unknown function (DUF3353) (DUF3353); Conserved in the Green Lineage 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACTCGCACTCGCCGCGTTCCCACATGAAGCGACAATGCCTCCCCTGC
Internal bar code:GTCGCCGGGAATTGCGAGATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3640
LEAP-Seq percent confirming:82.6087
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCGTGGTCACTGGGTAA
Suggested primer 2:AGAAATCCCATGATGCGCCA