| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006852 |
| Chromosome: | chromosome 7 |
| Location: | 2611841 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330200 | PF17,RSP9 | Radial Spoke Protein 9 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTAAGCGCAGAATCACTGACAGTACACAGGCATAAGACGGCACCTCT |
| Internal bar code: | CAAGGTCGCCTTAATGATGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1224 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAACAGCAGGGCAAAGG |
| Suggested primer 2: | GCATTAAGGGCATGCTCAGC |