Insertion junction: CLIP2.006856_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006856
Chromosome:chromosome 3
Location:3660674
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre50.g761497 (1 of 1) PTHR13890:SF0 - MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTCTCCCCCACCTACCCCCGCCTTCTGGAACCCCACACTGTCTTGGC
Internal bar code:TAGGCGCCGTCCCTGTCGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1036
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGACGCACCTGTCAAGAC
Suggested primer 2:CAGCAGCACGTCCTCCTTTA