| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006856 |
| Chromosome: | chromosome 3 |
| Location: | 3660675 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre50.g761497 | (1 of 1) PTHR13890:SF0 - MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGCGTGGCGCCTCCCCCCCTCGCCGCATGTGGGGCGCCACACCAGTG |
| Internal bar code: | ATATTTCTATCGGGATACACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 3.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCACGTCCTCCTTTA |
| Suggested primer 2: | TCAGACGCACCTGTCAAGAC |