Insertion junction: CLIP2.006859_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006859
Chromosome:chromosome 4
Location:3568214
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g228450 CPL10 (1 of 1) PTHR36716:SF2 - F3H9.20 PROTEIN; Conserved in the Plant Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTACCTGCATGGCGGCCCGTGTGGAGTCCTGGTCAGCGGGCAGCAGCC
Internal bar code:TTGTTATGGTCGAGTATTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:716
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTATGAGCTGGCTTACCGGG
Suggested primer 2:GTGCGTGAGGGGGATTACAA