| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006865 |
| Chromosome: | chromosome 3 |
| Location: | 5195520 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181500 | DPE1,STA11 | Disproportionating enzyme 1; (1 of 1) PTHR32438:SF5 - 4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCCGTAACCCTCACCCCCAGCCGTAACCCACCCGGATCGCCTTCCAC |
| Internal bar code: | GGGGAGTTTGTAAAATAAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3084 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCCGACGTTGACTATCC |
| Suggested primer 2: | CACCACTTGAAGCCCTCCTT |