| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006871 |
| Chromosome: | chromosome 1 |
| Location: | 6109473 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043350 | CAO1,CAO | Chlorophyllide a oxygenase, chloroplast precursor; (1 of 1) K13600 - chlorophyllide a oxygenase (CAO) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCAGTCGCGCGTGGAGCAGAACCTACAGCGCGTGAATGAGCTGAAG |
| Internal bar code: | ACTGTCAATAGATTCTAAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCCTCCTCAATCTCCAT |
| Suggested primer 2: | AGCTGAGCATGGATTGGCTT |