Insertion junction: CLIP2.006874_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006874
Chromosome:chromosome 6
Location:7425740
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g300500 ADCY1,CYA18 Adenylyl cyclase; (1 of 1) PTHR11347//PTHR11347:SF132 - CYCLIC NUCLEOTIDE PHOSPHODIESTERASE // PDE1C, ISOFORM E CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGGTGCGTGCGTGGTCGACTGCAGCCGGCTTACAAGCACTAGCAGGC
Internal bar code:GGATACATACTCTCCGTTGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3259
LEAP-Seq percent confirming:93.4783
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTGTCGAACTCCTGCTT
Suggested primer 2:TGTGCTGCTTACCCATCGTT