| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006895 |
| Chromosome: | chromosome 12 |
| Location: | 1955499 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511400 | CYCR3,PPR4 | PentatricoPeptide Repeat protein 4; (1 of 1) PTHR10177//PTHR24015 - CYCLINE // FAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGCAGCCAGCAGTACAGGCACACCATGGCCACGCCCGCGGTCATGG |
| Internal bar code: | CTGGAGGCCACAGCGAATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3819 |
| LEAP-Seq percent confirming: | 96.2366 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 186 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTACGGTCGGATATTGG |
| Suggested primer 2: | GTTCTCAAAGTGCTGCACCG |