Insertion junction: CLIP2.006904_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006904
Chromosome:chromosome 13
Location:298470
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre13.g564100 PTK2 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Protein tyrosine kinase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCATGTGAAGCAAAGCGTAGGCAATGCACAATAATCTCAAGAGATTG
Internal bar code:ACGACTCGTATATTCGGGTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATCTGACTGTGCGAACT
Suggested primer 2:ATGCCCACCAACGATGACAT