Insertion junction: CLIP2.006924_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006924
Chromosome:chromosome 12
Location:1748200
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g486100 CLPP5 active subunit of the chloroplast ClpP complex; (1 of 1) PTHR10381:SF12 - ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 5, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGAGCGGGCCGGCGCCTACGCCGCTGCACCAAAAGGGGGTGTACGAT
Internal bar code:GTACGCTCCGACGTGTATAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1119
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTGCTCACGATCGACTCC
Suggested primer 2:ACTAATCCTCCGCTCCCCTT