Insertion junction: CLIP2.006924_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.006924
Chromosome:chromosome 12
Location:1748201
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g486100 CLPP5 active subunit of the chloroplast ClpP complex; (1 of 1) PTHR10381:SF12 - ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 5, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACACGGAACTAAAAGCACACAGCACGCTGGACCGCCGTCTGCACCGC
Internal bar code:GTACGCTCCGACGTGTATAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:539
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACTAATCCTCCGCTCCCCTT
Suggested primer 2:CTCTGCTCACGATCGACTCC