| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006944 |
| Chromosome: | chromosome 2 |
| Location: | 7994466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145050 | CMK1,CMK | (1 of 1) 2.7.1.148 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase / CMK; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTAATCCTGCTGAGGCTTCGCCGGCAACAGTACGAGCCGGCAGCAGCA |
| Internal bar code: | AAAACACAGTTTGACGCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1886 |
| LEAP-Seq percent confirming: | 98.6667 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAAAACAGCGGCTGATCG |
| Suggested primer 2: | GGTTGCAAGGGACCCGATAA |