Insertion junction: CLIP2.006944_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006944
Chromosome:chromosome 2
Location:7994471
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g145050 CMK1,CMK (1 of 1) 2.7.1.148 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase / CMK; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplast precursor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGGGATATGAGGAGTTGTGGGATCATTTGCAACACAACGGTCCGTACA
Internal bar code:AAAACACAGTTTGACGCGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3158
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGCAAGGGACCCGATAA
Suggested primer 2:GCTAAAACAGCGGCTGATCG