Insertion junction: CLIP2.006946_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006946
Chromosome:chromosome 12
Location:2040741
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g510400 RBD4,CPLD30 Conserved in the Plant Lineage and Diatoms; (1 of 4) IPR024934 - Rubredoxin-like domain 3'UTR
Cre12.g801374 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGTGCTCAATGCACGATGCCGCACAGTACGATCAAGGACTCCATGCA
Internal bar code:TCTTCGGAGGAATCCTAATCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:5543
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCTGCCATGCACTTTG
Suggested primer 2:AGGCCCATATCCTCTGGTGT