| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006967 |
| Chromosome: | chromosome 16 |
| Location: | 5541272 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681900 | SECY1,SCY1 | Thylakoid translocase secY subunit; (1 of 1) PTHR10906//PTHR10906:SF9 - SECY/SEC61-ALPHA FAMILY MEMBER // PREPROTEIN TRANSLOCASE SUBUNIT SCY1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTCGCTGCTGGGCTCGGTGTTCCTGGGCGCCCTCAGCGCGGCCCCGG |
| Internal bar code: | TCAGAGTTATGAAGTACGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGGCTGTCCCTGTCAG |
| Suggested primer 2: | CATCTTCGCCTCCTCCATCC |