| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006973 |
| Chromosome: | chromosome 16 |
| Location: | 6445085 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675100 | CPLD53 | Conserved in the Plant Lineage and Diatoms; (1 of 3) PTHR31319:SF10 - ZINC FINGER PROTEIN CONSTANS-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCTTGGTGCCTCTGTGCCGCGTGCCCAGCAGCAGTGCCGGCGGCGGTG |
| Internal bar code: | AAGTTAAGTTAGTCAGTCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4598 |
| LEAP-Seq percent confirming: | 78.9474 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCTAAATCGCACCTGC |
| Suggested primer 2: | GCGGCCTTCCTTACCTTCTT |