Insertion junction: CLIP2.006977_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006977
Chromosome:chromosome 3
Location:2099428
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156500 (1 of 1) IPR000104//IPR016084 - Antifreeze protein, type I // Haem oxygenase-like, multi-helical intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGTCCTCATCAGCAGCACCTGTAGGAAATACCCGGCTGCCTGATATTG
Internal bar code:CTCGACAGACTATTCGATATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2415
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCACAAACACACCTCG
Suggested primer 2:AAGCCATGTCACCACCTAGC