| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006991 |
| Chromosome: | chromosome 12 |
| Location: | 8588915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548651 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACCACCAGCTGCAGCCGCAGCTCCAGCAGTAGTTCGGAGGGCGGCGG |
| Internal bar code: | GTTTGCTTACTAACTACTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1040 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCCATTGTACTCACAGC |
| Suggested primer 2: | CGTTTGGCCTTCACATTGCA |