| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006995 |
| Chromosome: | chromosome 9 |
| Location: | 2575130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392500 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCACCTCCAAGCCCAGCTCTCATCAGAAGTGCCTTGACATATTTTAAT |
| Internal bar code: | CTTTGGGCCAGTATATGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGCTTGCTGAACTTGC |
| Suggested primer 2: | CCTCAGGCCCACCATTGATT |