Insertion junction: CLIP2.007000_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.007000
Chromosome:chromosome 3
Location:637193
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145587 CPLD1 Conserved in the Plant Lineage and Diatoms; (1 of 4) PF04134 - Protein of unknown function, DUF393 (DUF393) 3'UTR
Cre03.g145607 BET3 (1 of 1) IPR016721 - Bet3-like protein; Component of TRAPP complex outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCAATGCGCCCTCAGTGCACTGGTTGAAGACGGTATGTCAACCTTCAA
Internal bar code:TTACGGTATTACAGTGCTTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1213
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACGACGTGTGGGCATCAAA
Suggested primer 2:TATGTGCGGTCTGGGTATGC