| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007025 |
| Chromosome: | chromosome 2 |
| Location: | 7512497 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392729 | (1 of 2) 2.1.2.9 - Methionyl-tRNA formyltransferase / transformylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCAGTAGCAGCAGCGACAGCAGAAGTAGCCGCAGCAGCAGTAGCAGC |
| Internal bar code: | TATAGGACGCAGGTGTAAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5251 |
| LEAP-Seq percent confirming: | 98.6301 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTCTTCGCAATCGCACG |
| Suggested primer 2: | ACTTCTCGTGCTTCCCACTG |