Insertion junction: CLIP2.007025_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007025
Chromosome:chromosome 2
Location:7512506
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g392729 (1 of 2) 2.1.2.9 - Methionyl-tRNA formyltransferase / transformylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTGCCAGTGCTGCCACTGCCGCCGGGCGTGCCGGTGCCTGTGCAAACC
Internal bar code:TAGAGAGTTCGCAGAGTGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3770
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCTCGTGCTTCCCACTG
Suggested primer 2:CTTCTCTTCGCAATCGCACG