| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.007026 |
| Chromosome: | chromosome 13 |
| Location: | 2433306 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579550 | CGL27 | Conserved in the Green Lineage; (1 of 5) IPR002645 - STAS domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATGACTCTTCCGACGCCGCGCCCAGCGCACCGCACGTAGCAATGTC |
| Internal bar code: | TTTGAGACTCCTGCGCATCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGACCGTGGTGTAAGAGG |
| Suggested primer 2: | TGTTGCTCCTGATCCTGCTG |