Insertion junction: CLIP2.007051_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007051
Chromosome:chromosome 6
Location:6635899
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g294750 CHLG1,CHLG Chlorophyll synthetase; (1 of 1) 2.5.1.62 - Chlorophyll synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTACTGATACGTGCTCTGACACGTGTTTTAGGGCGGGTTGCAGGTTAG
Internal bar code:TAGTTTTTGCAAACAGAATTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3616
LEAP-Seq percent confirming:96.9697
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCCAAGGGGTGCTTCAT
Suggested primer 2:GAGCCCACTTGGACTGACTC