| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007053 |
| Chromosome: | chromosome 3 |
| Location: | 6193202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191300 | LCI34,LCI35 | Low-CO2-inducible protein 35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACGATGCCGGCGTGCGTACACACGCGTGTGCAAGACGGGAAGTGCTG |
| Internal bar code: | CCTCATGCTTTGACCTAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 3.44828 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAATCCTTGCGCATCTGC |
| Suggested primer 2: | CTCCATGGATCTGGACACGG |